G3F5 group resulted in the highest motility, mitochondrial activity and viability and the lowest DNA fragmentation and DNA damage (p<0.05). Semen samples collected from six mature rams were pooled and splitted into equal aliquots and diluted with a tris‐based extender containing different concentrations of glycerol (G5 %5 and G3 %3), fetuin (F 2.5, 5, and 15 mg/ml), and trehalose (60 mM) as eight groups (G5F0, G5F2.5, G5F5, G5F15, G3F0, G3F2.5, G3F5, and G3F15). The aim of this study was to determine the effects of fetuin and trehalose added into ram semen extender on sperm parameters, antioxidant paremeters, antioxidant‐related gene expressions and DNA integrity during the freeze‐thawing process, in low glycerol concentration. Thus, antioxidant supplementation of sperm during freeze‐thawing is suggested to improve sperm parameters. Perhaps this is the next technological revolution, the advent of large-scale and complex Cyber-physical Cognitive systems.įreeze‐thawing process negatively affects ram sperm in terms of sperm quality, DNA integrity and antioxidant defence system. While, on one hand, the isolated application of the cyber systems starts to be wild spread, on the other hand, their synchronically integration with other cyber system to build a concise and cognitive structure that can interact deeply and autonomously with a physical system is still a completely open question, only addressed in some works from the philosophical point of view. All those developments are recent and have the potential to change the future of society. Those developments are allowing the emergence of new Cyber Systems where the data constantly generated are leveraged to build Artificial Intelligence models to perform specialized task. In part, these changes have been fomented by the new technologies, which are providing high performance computing capacities, cloud computing, almost instantly change of information world widely, big-data pipelines that enable the creation of complex Artificial Intelligence systems. Modern society is living a moment of paradigms changes. Hence, a protocol has been developed to allow frozen-thawed bull sperm to be sex-sorted with high resolution between the sexes, then re-frozen and thawed with retention of motility and acrosome integrity. Sperm were more motile (58.2 +/- 4.7 vs 38.7 +/- 3.5 p < 0.001) and had better acrosome integrity (74.3 +/- 2.9 vs 66.8 +/- 2.0 p < 0.001) after freezing in an extender containing 0.375% Equex STM Paste than in extender without Equex. Sperm orientation (p < 0.05) and motility (69.9 +/- 3.0 vs 55.6 +/- 4.0 p < 0.001) were highest after staining in Androhep rather than in TALP buffer. Frozen-thawed sperm were better orientated (p = 0.006) and had fewer damaged membranes (8.7 +/- 0.6% vs 19.5 +/- 2.4% p = 0.003) after centrifugation in PureSperm rather than BoviPure gradients. Sperm were sorted and collected into Test yolk buffer, and frozen in an extender containing 0, 0.25, 0.375 or 0.5% Equex STM Paste. Sperm were prepared for sorting by density gradient separation in either PureSperm or BoviPure, followed by staining in one of three diluents (Androhep, Bovine Sheath Fluid + 0.3% BSA or TALP buffer). This technique is attractive to the dairy industry, and therefore a series of three experiments was designed to investigate the optimal methods to prepare, sex-sort and re-freeze frozen-thawed bovine sperm. A successful method used to overcome this limitation in sheep is sex-sorting from frozen-thawed semen and refreezing for artificial insemination. One drawback to the technology is that donor bulls must be located within a short distance of the sorting facility in order to collect semen, which limits the number of bulls from which sorted sperm are available. Dairy bull sperm may be sex-sorted, frozen and used to artificially inseminate heifers with acceptable fertility if the herd is well-managed.
0 Comments
Leave a Reply. |